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, Annexe 3.2 : Structure d'une banque d'ADNc pour le séquençage Illumina en paired-end
, Annexe 3.3 : Rappel de la technologie de séquençage haut-débit d'Illumina
, Les adaptateurs sont attachés aux extrémités des fragments de séquence
, PCR par pont" amplifient chaque fragment attaché à la flow cell pour produire des clusters, Les réactions
, Une seule base est incorporée à chaque cycle PCR par l'utilisation de nucléotides modifiés
) l'ajout d'une base, (2) l'acquisition d'image, (3) le clivage chimique du groupement "terminator" et le marquage fluorescent pour le cycle suivant ,
, Extrait du descriptif d'un read de l'individu Ag19 (Arillastrum gummiferum) dans un fichier .FASTQ. LOC104436372 pectinesterase-like 3,574701001 ARIGU_g124210:1:1432:2:+ Pectinesterase 3, vol.3, p.65728359
, LOC104436372 pectinesterase-like 3,574701001 ARIGU_g124210:1:1432:2:+ Pectinesterase 3, vol.3, p.65728359
, LOC104436373 pectinesterase-like 3,735241395 ARIGU_g124210:1:1432:2:+ Pectinesterase 3, vol.3, p.65728359
, LOC104439065 anthocyanidin 3-O-glucosyltransferase 7 2,505487508 ARIGU_g231350:1:1550:3:+ anthocyanidin 3-O-glucosyltransferase 7-like, vol.2, p.84116646
, LOC104451753 anthocyanidin 3-O-glucosyltransferase 7 3,142882133 ARIGU_g231350:1:1550:3:+ anthocyanidin 3-O-glucosyltransferase 7-like, vol.2, p.84116646
, LOC104451753 anthocyanidin 3-O-glucosyltransferase 7 3,142882133 ARIGU_g231350:1:1550:3:+ anthocyanidin 3-O-glucosyltransferase 7-like, vol.2, p.84116646
, LOC104451782 anthocyanidin 3-O-glucosyltransferase 7 2,795907991 ARIGU_g231350:1:1550:3:+ anthocyanidin 3-O-glucosyltransferase 7-like, vol.2, p.84116646
, Annotation fonctionnelle et positions des 3 SNPs discriminants localisés dans des gènes différentiellement exprimés chez A. gummiferum
, , p.21098916
, , p.21098916
, Annotation fonctionnelle et positions des 68 SNPs discriminants localisés dans des gènes différentiellement exprimés chez S. longifolium (In-house). En jaune sont indiqués les SNPs communs avec la méthode GATK, vol.5
, Annotation fonctionnelle du gène différentiellement exprimé et contenant un SNP discriminant chez T. glauca-BDS, vol.6, p.34161705