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Screening New Xylanase Biocatalysts from the Mangrove Soil Diversity

Abstract : Mangrove sediments from New Caledonia were screened for xylanase sequences. One enzyme was selected and characterized both biochemically and for its industrial potential. Using a specific cDNA amplification method coupled with a MiSeq sequencing approach, the diversity of expressed genes encoding GH11 xylanases was investigated beneath Avicenia marina and Rhizophora stylosa trees during the wet and dry seasons and at two different sediment depths. GH11 xylanase diversity varied more according to tree species and season, than with respect to depth. One complete cDNA was selected (OFU29) and expressed in Pichia pastoris. The corresponding enzyme (called Xyn11-29) was biochemically characterized, revealing an optimal activity at 40–50 °C and at a pH of 5.5. Xyn11-29 was stable for 48 h at 35 °C, with a half-life of 1 h at 40 °C and in the pH range of 5.5–6. Xyn11-29 exhibited a high hydrolysis capacity on destarched wheat bran, with 40% and 16% of xylose and arabinose released after 24 h hydrolysis. Its activity on wheat straw was lower, with a release of 2.8% and 6.9% of xylose and arabinose, respectively. As the protein was isolated from mangrove sediments, the effect of sea salt on its activity was studied and discussed.
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https://hal-insu.archives-ouvertes.fr/insu-03293424
Contributeur : Nathalie Pothier <>
Soumis le : mercredi 21 juillet 2021 - 09:07:14
Dernière modification le : mardi 3 août 2021 - 03:38:39

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microorganisms-09-01484-v2.pdf
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Distributed under a Creative Commons Paternité 4.0 International License

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Corinne Ivaldi, Mariane Daou, Laurent Vallon, Alexandra Bisotto, Mireille Haon, et al.. Screening New Xylanase Biocatalysts from the Mangrove Soil Diversity. Microorganisms, MDPI, 2021, 9 (7), pp.1-16. ⟨10.3390/microorganisms9071484⟩. ⟨insu-03293424⟩

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